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Optics Letters

Optics Letters


  • Vol. 23, Iss. 11 — Jun. 1, 1998
  • pp: 817–819

Quantitative optical phase microscopy

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts  »View Author Affiliations

Optics Letters, Vol. 23, Issue 11, pp. 817-819 (1998)

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We present a new method for the extraction of quantitative phase data from microscopic phase samples by use of partially coherent illumination and an ordinary transmission microscope. The technique produces quantitative images of the phase profile of the sample without phase unwrapping. The technique is able to recover phase even in the presence of amplitude modulation, making it significantly more powerful than existing methods of phase microscopy. We demonstrate the technique by providing quantitatively correct phase images of well-characterized test samples and show that the results obtained for more-complex samples correlate with structures observed with Nomarski differential interference contrast techniques.

© 1998 Optical Society of America

OCIS Codes
(000.2170) General : Equipment and techniques
(100.0100) Image processing : Image processing
(100.3010) Image processing : Image reconstruction techniques
(110.0180) Imaging systems : Microscopy
(180.0180) Microscopy : Microscopy

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, "Quantitative optical phase microscopy," Opt. Lett. 23, 817-819 (1998)

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  1. M. R. Teague, J. Opt. Soc. Am. 73, 1434 (1983).
  2. T. E. Gureyev and K. A. Nugent, Opt. Commun. 133, 339 (1997).
  3. D. Paganin and K. A. Nugent, Phys. Rev. Lett. 80, 2586 (1998).
  4. S. T. Huntington, P. Mulvaney, A. Roberts, and K. A. Nugent, J. Appl. Phys. 86, 1 (1997).

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