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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 23, Iss. 14 — Jul. 15, 1998
  • pp: 1111–1113

Near-field scanning optical microscopy of single molecules by femtosecond two-photon excitation

Michael K. Lewis, Peter Wolanin, Ari Gafni, and Duncan G. Steel  »View Author Affiliations


Optics Letters, Vol. 23, Issue 14, pp. 1111-1113 (1998)
http://dx.doi.org/10.1364/OL.23.001111


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Abstract

We present a demonstration of near-field scanning optical microscopy of single molecules based on ultrafast two-photon-induced fluorescence. Measurements were performed by use of 100-fs pulses at 800 nm from a Ti:sapphire laser to excite the two-photon transition in Rhodamine B molecules. Although near-field probes are normally metal coated to achieve superresolution, we used uncoated tips to achieve sufficiently high optical powers to generate acceptable fluorescence emission rates. Images of single molecules demonstrate a resolution of ~175nm(< λ/4) on a topographically smooth surface, which surpasses the apparent λ/2 resolution limit for uncoated tips operating in the linear response regime.

© 1998 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(260.2510) Physical optics : Fluorescence

Citation
Michael K. Lewis, Peter Wolanin, Ari Gafni, and Duncan G. Steel, "Near-field scanning optical microscopy of single molecules by femtosecond two-photon excitation," Opt. Lett. 23, 1111-1113 (1998)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-23-14-1111

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