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Optics Letters

Optics Letters


  • Vol. 23, Iss. 15 — Aug. 1, 1998
  • pp: 1221–1223

Three-dimensional microscopy with phase-shifting digital holography

Tong Zhang and Ichirou Yamaguchi  »View Author Affiliations

Optics Letters, Vol. 23, Issue 15, pp. 1221-1223 (1998)

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We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.

© 1998 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(100.0100) Image processing : Image processing
(180.0180) Microscopy : Microscopy

Tong Zhang and Ichirou Yamaguchi, "Three-dimensional microscopy with phase-shifting digital holography," Opt. Lett. 23, 1221-1223 (1998)

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  1. D. A. Agard, Annu. Rev. Biophys. Bioeng. 13, 191 (1984).
  2. T. Wilson, ed., Confocal Microscopy (Academic, London, 1990).
  3. D. Gabor, Proc. R. Soc. London Ser. A 197, 454 (1949).
  4. R. F. van Ligten, Nature (London) 211, 282 (1966).
  5. R. F. van Ligten, Opt. Technol. 1, 71 (1969).
  6. T.-C. Poon, K. B. Doh, B. W. Schiling, M. H. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
  7. W. S. Bradley and T.-C. Poon, Opt. Lett. 19, 1506 (1997).
  8. K. B. Doh, T.-C. Poon, and G. Indebetouw, Opt. Eng. 35, 1550 (1996).
  9. E. Leigh, C. Chen, Y. Chen, D. Dilworth, J. Lopes, J. Rudd, P.-C. Sun, J. Valdmanis, and G. Vossler, J. Opt. Soc. Am. A 9, 1148 (1992).
  10. S. C. W. Hyde, N. P. Barry, R. Jones, J. C. Dainty, and P. M. W. French, Opt. Lett. 20, 1331 (1995).
  11. J. C. Marron, and K. S. Schroeder, Appl. Opt. 31, 255 (1992).
  12. U. Schnars, T. M. Kreis, and W. P. O. Juptner, Opt. Eng. 35, 977 (1996).
  13. I. Yamaguchi and T. Zhang, Opt. Lett. 22, 1268 (1997).
  14. J. Kato, I. Yamaguchi, T. Nakamura, and S. Kuwashima, Appl. Opt. 36, 8403 (1997).

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