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Optics Letters

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  • Vol. 23, Iss. 4 — Feb. 15, 1998
  • pp: 307–309

Optical measurement of the viscoelastic and biochemical responses of living cells to mechanical perturbation

Chau-Hwang Lee, Chin-Lin Guo, and Jyhpyng Wang  »View Author Affiliations


Optics Letters, Vol. 23, Issue 4, pp. 307-309 (1998)
http://dx.doi.org/10.1364/OL.23.000307


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Abstract

We have developed an optical method for real-time monitoring of cellular motion in a natural environment with nanometer resolution. From the motion driven by small optical forces, we measured dynamic viscoelastic responses of living cells in the linear reversible region. Cytoplasmic gel-to-sol transition that was due to the disruption of the actin-filament framework was detected, and a linear release of Ca2+ from intracellular storage that was related to submicrometer cell deformation was observed. The method was shown to be a powerful tool for studying the natural response of cells to mechanical perturbation.

© 1998 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy

Citation
Chau-Hwang Lee, Chin-Lin Guo, and Jyhpyng Wang, "Optical measurement of the viscoelastic and biochemical responses of living cells to mechanical perturbation," Opt. Lett. 23, 307-309 (1998)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-23-4-307


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