OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 23, Iss. 4 — Feb. 15, 1998
  • pp: 307–309

Optical measurement of the viscoelastic and biochemical responses of living cells to mechanical perturbation

Chau-Hwang Lee, Chin-Lin Guo, and Jyhpyng Wang  »View Author Affiliations

Optics Letters, Vol. 23, Issue 4, pp. 307-309 (1998)

View Full Text Article

Acrobat PDF (274 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We have developed an optical method for real-time monitoring of cellular motion in a natural environment with nanometer resolution. From the motion driven by small optical forces, we measured dynamic viscoelastic responses of living cells in the linear reversible region. Cytoplasmic gel-to-sol transition that was due to the disruption of the actin-filament framework was detected, and a linear release of Ca2+ from intracellular storage that was related to submicrometer cell deformation was observed. The method was shown to be a powerful tool for studying the natural response of cells to mechanical perturbation.

© 1998 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy

Chau-Hwang Lee, Chin-Lin Guo, and Jyhpyng Wang, "Optical measurement of the viscoelastic and biochemical responses of living cells to mechanical perturbation," Opt. Lett. 23, 307-309 (1998)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J. D. Watson, Molecular Biology of the Cell, 2nd ed. (Garland, New York, 1989), Chap. 11.
  2. T. Yamazaki, I. Komuro, and Y. Yazaki, J. Mol. Cell Cardiol. 27, 133 (1995).
  3. C.-H. Lee and J. Wang, Opt. Commun. 135, 233 (1997).
  4. See, for example, T. Wilson, ed., Confocal Microscopy (Academic, London, 1990).
  5. W. H. Press, S. A. Teukolsky, W. T. Vetterling, and B. P. Flannery, Numerical Recipes in C, 2nd ed. (Cambridge U. Press, Cambridge, 1992), Chap. 15.
  6. S. W. Tanenbaum, ed., Cytochalasins, Biochemical and Cell Biological Aspects (North-Holland, Amsterdam, 1978).
  7. J. A. Cooper, J. Cell Biol. 105, 1473 (1987).
  8. T. P. Stossel, Science 260, 1086 (1993).
  9. A. Ashkin and J. M. Dziedzic, Proc. Natl. Acad. Sci. USA 86, 7914 (1989).
  10. K. S. Zaner and P. A. Valberg, J. Cell Biol. 109, 2233 (1989).
  11. E. Evans, Methods Enzymol. 173, 3 (1989).
  12. N. O. Petersen, W. B. McConnaughey, and E. L. Elson, Proc. Natl. Acad. Sci. USA 79, 5327 (1982).
  13. A. Bearden, M. P. O'Neill, L. C. Osborne, and T. L. Wong, Opt. Lett. 18, 238 (1993).
  14. R. Juskaitis, T. Wilson, and N. P. Rea, Opt. Commun. 109, 167 (1994).
  15. A. Weeds, Nature (London) 296, 811 (1982).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited