OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 23, Iss. 7 — Apr. 1, 1998
  • pp: 555–557

Separation of changes in light scattering and chromophore concentrations during cortical spreading depression in rats

Matthias Kohl, Ute Lindauer, Ulrich Dirnagl, and Arno Villringer  »View Author Affiliations

Optics Letters, Vol. 23, Issue 7, pp. 555-557 (1998)

View Full Text Article

Acrobat PDF (265 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We used near-infrared spectroscopy to separate tissue scattering changes from changes in cerebral oxyhemoglobin and deoxyhemoglobin and the redox state of cytochrome- c -oxidase. A separate term of the transport scattering coefficient (µs) was included in a modified Lambert–Beer equation. It is shown by diffusion equation analysis that there is a simple relationship between the differential path-length factor (Da) and its scattering equivalent (Ds) . The method was applied to cortical spreading depression (CSD) data recorded through the skulls of rats. Biphasic changes in µsof ±0.1mm-1were observed during CSD’s that spread with a velocity of ~5mm/min . The method proposed has the promise to permit monitoring of scattering changes noninvasively in humans during cortical activation or pathophysiological conditions.

© 1998 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5280) Medical optics and biotechnology : Photon migration
(170.6930) Medical optics and biotechnology : Tissue

Matthias Kohl, Ute Lindauer, Ulrich Dirnagl, and Arno Villringer, "Separation of changes in light scattering and chromophore concentrations during cortical spreading depression in rats," Opt. Lett. 23, 555-557 (1998)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. F. F. Jöbsis, Science 198, 1264 (1977).
  2. D. T. Delpy, M. Cope, P. van der Zee, S. R. Arridge, S. Wray, and J. S. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
  3. R. A. Stepnoski, A. LaPorta, F. Raccuia-Behling, G. E. Blonder, R. E. Slusher, and D. Kleinfeld, Proc. Natl. Acad. Sci. USA 88, 9382 (1991).
  4. D. Malonek and A. Grinvald, Science 272, 551 (1996).
  5. G. Gratton, P. M. Corballis, E. Cho, M. Fabiani, and D. C. Hood, Psychophysiology 32, 505 (1995).
  6. M. S. Patterson, B. Chance, and B. C. Wilson, Appl. Opt. 28, 2331 (1989).
  7. J. B. Fishkin, P. T. C. So, A. E. Cerussi, S. Fantini, M. A. Franceschini, and E. Gratton, Appl. Opt. 34, 1143 (1995).
  8. S. J. Masden, E. R. Anderson, R. C. Haskell, and B. J. Tromberg, Opt. Lett. 19, 1934 (1994).
  9. M. Kohl, R. Watson, and M. Cope, Opt. Lett. 21, 1519 (1996).
  10. T. Wolf, U. Lindauer, H. Obrig, J. Dreier, T. Back, A. Villringer, and U. Dirnagl, J. Cereb. Blood Flow Metab. 16, 1100 (1996).
  11. M. Nedergaard and A. J. Hansen, J. Cereb. Blood Flow Metab. 13, 568 (1993).
  12. M. Lauritzen, Brain 117, 199 (1994).
  13. S. J. Matcher, M. Cope, and D. T. Delpy, Appl. Opt. 36, 386 (1997).
  14. R. Graff, J. G. Aarnoudse, J. R. Ziip, P. M. A. Sloot, F. F. M. de Mul, J. Greve, and M. H. Koelink, Appl. Opt. 31, 1370 (1992).
  15. S. J. Matcher, C. E. Elwell, C. E. Cooper, M. Cope, and D. T. Delpy, Anal. Biochem. 227, 54 (1995).
  16. M. Essenpreis, C. E. Elwell, M. Cope, P. van der Zee, S. R. Arridge, and D. T. Delpy, Appl. Opt. 32, 418 (1993).
  17. A. Kienle and M. S. Patterson, J. Opt. Soc. Am. A 14, 246 (1997).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited