We applied photoacoustics as a tissue tomography technique for the detection of blood concentrations, e.g., angiogenesis around tumors. We imaged blood vessels in highly scattering samples, using 532-nm light, to depths of ~1 cm . The samples were real tissue (chicken breast) or 10% dilutions of Intralipid-10%. The blood flowed through nylon capillaries. Polyvinylidene difluoride (PVdF) piezoelectric detectors were used in a surface-scanning mode. We demonstrate the sensitivity of the technique by photoacoustic detection of single red blood cells upon a glass plate. Lateral resolution is limited by the detector diameter (200 µm). The depth resolution is ~10 µm.
© 1998 of America
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1870) Medical optics and biotechnology : Dermatology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6930) Medical optics and biotechnology : Tissue
C. G. A. Hoelen, F. F. M. de Mul, R. Pongers, and A. Dekker, "Three-dimensional photoacoustic imaging of blood vessels in tissue," Opt. Lett. 23, 648-650 (1998)