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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 23, Iss. 9 — May. 1, 1998
  • pp: 655–657

Multifocal multiphoton microscopy

Jörg Bewersdorf, Rainer Pick, and Stefan W. Hell  »View Author Affiliations


Optics Letters, Vol. 23, Issue 9, pp. 655-657 (1998)
http://dx.doi.org/10.1364/OL.23.000655


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Abstract

We present a real-time, direct-view multiphoton excitation fluorescence microscope that provides three-dimensional imaging at high resolution. Using a rotating microlens disk, we split the near-infrared light of a mode-locked titanium–sapphire laser into an array of beams that are transformed into an array of high-aperture foci at the object. We typically scan at 225 frames per second and image the fluorescence with a camera that reads out the images at video rate. For 1.4 aperture oil and 1.2 water immersion lenses at 780-nm excitation we obtained axial resolutions of 0.84 and 1.4μm , respectively, which are similar to that of a single-beam two-photon microscope. Compared with the latter setup, our system represents a 40–100-fold increase in efficiency, or imaging speed. Moreover, it permits the observation with the eye of high-resolution two-photon images of (live) samples.

© 1998 Optical Society of America

OCIS Codes
(100.6890) Image processing : Three-dimensional image processing
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes

Citation
Jörg Bewersdorf, Rainer Pick, and Stefan W. Hell, "Multifocal multiphoton microscopy," Opt. Lett. 23, 655-657 (1998)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-23-9-655


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References

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