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Optics Letters

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  • Vol. 24, Iss. 2 — Jan. 15, 1999
  • pp: 116–118

Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser

Yong-le Pan, Stephen Holler, Richard K. Chang, Steven C. Hill, Ronald G. Pinnick, Stanley Niles, and Jerold R. Bottiger  »View Author Affiliations


Optics Letters, Vol. 24, Issue 2, pp. 116-118 (1999)
http://dx.doi.org/10.1364/OL.24.000116


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Abstract

Reproducible fluorescence spectra of individual 2- to 5-µm -diameter biological aerosol particles excited with a single shot from a Q -switched laser (266 or 351 nm) have been obtained with highly improved signal-to-noise ratios. Critical to the advance are crossed diode-laser trigger beams, which precisely define the sample volume, and a reflecting objective, which minimizes chromatic aberration and has a large N.A. for collecting fluorescence. Several allergens (red oak, meadow oat pollen, paper mulberry pollen, and puffball spores) have different fluorescence spectra. Bacillus subtilis fluorescence spectrum deteriorates at high 266-nm incident intensity. Dry riboflavin particles illuminated with a 351-nm light exhibit a new 420-nm fluorescence peak that grows nonlinearly with laser pulse energy.

© 1999 Optical Society of America

OCIS Codes
(010.1110) Atmospheric and oceanic optics : Aerosols
(140.3610) Lasers and laser optics : Lasers, ultraviolet
(260.2510) Physical optics : Fluorescence
(300.6170) Spectroscopy : Spectra

Citation
Yong-le Pan, Stephen Holler, Richard K. Chang, Steven C. Hill, Ronald G. Pinnick, Stanley Niles, and Jerold R. Bottiger, "Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser," Opt. Lett. 24, 116-118 (1999)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-24-2-116


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References

  1. M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. (to be published).
  2. P. P. Hairston, J. Ho, and F. R. Quant. J. Aerosol Sci. 28, 471 (1997).
  3. R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. I. Fernadez, M. W. Mayo, and J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
  4. G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, Opt. Lett. 21, 1307 (1996).
  5. R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, Aerosol Sci. Technol. 28, 95 (1998).
  6. The problem of using fluorescence to classify atmospheric bioaerosols is challenging because (1) there are so many possible types of biological aerosol particle, (2) differences between the emission spectra of highly purified bacterial or protein samples may be small (spectra may be dominated by a small number of primary fluorophors, e.g., aromatic amino acids, reduced nicotinamide compounds, and flavins), (3) differences may depend on growth conditions, the atmospheric environment (temperature, humidity, sunlight, etc.), and other factors, and (4) naturally occurring biological aerosols may be mixtures of several types of particle and compound.
  7. S. Holler, Y. L. Pan, R. K. Chang, S. C. Hill, D. B. Hillis, and J. R. Bottiger, Opt. Lett. 23, 1489 (1998).
  8. G. W. Faris, R. A. Copeland, K. Mortelmans, and B. V. Bronk, Appl. Opt. 36, 958 (1997).
  9. M. Seaver, D. C. Roselle, J. G. Pinto, and J. D. Eversole, Appl. Opt. 37, 5344 (1998).

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