Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity
Optics Letters, Vol. 24, Issue 24, pp. 1832-1834 (1999)
http://dx.doi.org/10.1364/OL.24.001832
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Abstract
We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes.
© 1999 Optical Society of America
[Optical Society of America ]
OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes
Citation
Richard A. Farrer, Michael J. R. Previte, Christopher E. Olson, Lynn Anne Peyser, John T. Fourkas, and Peter T. C. So, "Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity," Opt. Lett. 24, 1832-1834 (1999)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-24-24-1832
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