By varying the chirp of high-intensity pulses, we can use the chirp-condition-dependent fluorescence yield to distinguish among different molecules or the same molecule in different microenvironments. As an example of the latter we show that SNAFL-2, a well-known pH-sensitive dye, shows large modulation in fluorescence yield in response to both variation in acidity and variation in chirp condition. Future application of this technique as a novel contrast mechanism within fluorescence microscopy is discussed.
© 1999 Optical Society of America
(050.1590) Diffraction and gratings : Chirping
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence
(320.2250) Ultrafast optics : Femtosecond phenomena
(320.7130) Ultrafast optics : Ultrafast processes in condensed matter, including semiconductors
Arjan H. Buist, Michiel Mller, Rick I. Ghauharali, G. J. Brakenhoff, Jeff A. Squier, Chris J. Bardeen, Vladislav V. Yakovlev, and Kent R. Wilson, "Probing microscopic chemical environments with high-intensity chirped pulses," Opt. Lett. 24, 244-246 (1999)