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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 25, Iss. 1 — Jan. 1, 2000
  • pp: 46–48

Lateral resolution enhancement with standing evanescent waves

George E. Cragg and Peter T. C. So  »View Author Affiliations


Optics Letters, Vol. 25, Issue 1, pp. 46-48 (2000)
http://dx.doi.org/10.1364/OL.25.000046


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Abstract

A high-resolution fluorescence microscopy technique has been developed that achieves a lateral resolution of better than one sixth of the emission wavelength (FWHM). By use of a total-internal-reflection geometry, standing evanescent waves are generated that spatially modulate the excitation of the sample. An enhanced two-dimensional image is formed from a weighted sum of images taken at different phases and directions of the standing wave. The performance of such a system is examined through theoretical calculations of both the point-spread function and the optical transfer function.

© 2000 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy
(180.5810) Microscopy : Scanning microscopy

Citation
George E. Cragg and Peter T. C. So, "Lateral resolution enhancement with standing evanescent waves," Opt. Lett. 25, 46-48 (2000)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-25-1-46

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