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Optics Letters

Optics Letters


  • Vol. 25, Iss. 1 — Jan. 1, 2000
  • pp: 52–54

Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy

O. Albert, L. Sherman, G. Mourou, T. B. Norris, and G. Vdovin  »View Author Affiliations

Optics Letters, Vol. 25, Issue 1, pp. 52-54 (2000)

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Off-axis aberrations in a beam-scanning multiphoton confocal microscope are corrected with a deformable mirror. The optimal mirror shape for each pixel is determined by a genetic learning algorithm, in which the second-harmonic or two-photon fluorescence signal from a reference sample is maximized. The speed of the convergence is improved by use of a Zernike polynomial basis for the deformable mirror shape. This adaptive optical correction scheme is implemented in an all-reflective system by use of extremely short (10-fs) optical pulses, and it is shown that the scanning area of an f:1 off-axis parabola can be increased by nine times with this technique.

© 2000 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

O. Albert, L. Sherman, G. Mourou, T. B. Norris, and G. Vdovin, "Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy," Opt. Lett. 25, 52-54 (2000)

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