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Optics Letters

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  • Vol. 25, Iss. 18 — Sep. 15, 2000
  • pp: 1361–1363

Whole-field optically sectioned fluorescence lifetime imaging

M. J. Cole, J. Siegel, S. E. D. Webb, R. Jones, K. Dowling, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. A. Neil, R. Juškaitis, and T. Wilson  »View Author Affiliations


Optics Letters, Vol. 25, Issue 18, pp. 1361-1363 (2000)
http://dx.doi.org/10.1364/OL.25.001361


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Abstract

We describe a novel three-dimensional fluorescence lifetime imaging microscope that exploits structured illumination to achieve whole-field sectioned fluorescence lifetime images with a spatial resolution of a few micrometers.

© 2000 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
M. J. Cole, J. Siegel, S. E. D. Webb, R. Jones, K. Dowling, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. A. Neil, R. Juškaitis, and T. Wilson, "Whole-field optically sectioned fluorescence lifetime imaging," Opt. Lett. 25, 1361-1363 (2000)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-25-18-1361


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References

  1. Q. S. Hanley, P. J. Ververer, D. J. Arndt-Jovin, and T. M. Jovin, J. Microsc. 197, 5 (2000).
  2. M. A. A. Neil, R. Juškaitis, and T. Wilson, Opt. Lett. 22, 1905 (1997).
  3. M. A. A. Neil, A. Squire, R. Juškaitis, P. I. H. Bastiaens, and T. Wilson, J. Microsc. 197, 1 (2000).
  4. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983).
  5. H. Szmacinski, J. R. Lakowicz, and M. L. Johnson, Methods Enzymol. 240, 723 (1994).
  6. P. C. Schneider and R. M. Clegg, Rev. Sci. Instrum. 68, 4107 (1997).
  7. R. Cubbedu, P. Taroni, and G. Valentini, Opt. Eng. 32, 320 (1993).
  8. J. Systma, J. M. Vroom, C. J. De Grauw, and H. C. Gerritsen, J. Microsc. 191, 39 (1998).
  9. A. Squire and P. I. H. Bastiaens, J. Microsc. 193, 36 (1999).
  10. M. Straub and S. W. Hell, Appl. Phys. Lett. 73, 1769 (1998).
  11. K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, Opt. Lett. 23, 810 (1998).
  12. K. Dowling, M. J. Dayel, S. C. W. Hyde, P. M. W. French, M. J. Lever, J. D. Hares, and A. K. L. Dymoke-Bradshaw, J. Mod. Opt. 46, (1999).
  13. R. Jones, K. Dowling, M. J. Cole, D. Parsons-Karavassilis, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, Electron. Lett. 35, 256 (1999).

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