OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 25, Iss. 22 — Nov. 15, 2000
  • pp: 1648–1650

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

G. Valentini, C. D'Andrea, D. Comelli, A. Pifferi, P. Taroni, A. Torricelli, R. Cubeddu, C. Battaglia, C. Consolandi, G. Salani, L. Rossi-Bernardi, and G. De Bellis  »View Author Affiliations


Optics Letters, Vol. 25, Issue 22, pp. 1648-1650 (2000)
http://dx.doi.org/10.1364/OL.25.001648


View Full Text Article

Acrobat PDF (517 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

© 2000 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(260.2510) Physical optics : Fluorescence

Citation
G. Valentini, C. D'Andrea, D. Comelli, A. Pifferi, P. Taroni, A. Torricelli, R. Cubeddu, C. Battaglia, C. Consolandi, G. Salani, L. Rossi-Bernardi, and G. De Bellis, "Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity," Opt. Lett. 25, 1648-1650 (2000)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-25-22-1648

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited