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Optics Letters

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  • Vol. 25, Iss. 24 — Dec. 15, 2000
  • pp: 1768–1770

Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration

Mamoru Hashimoto, Tsutomu Araki, and Satoshi Kawata  »View Author Affiliations


Optics Letters, Vol. 25, Issue 24, pp. 1768-1770 (2000)
http://dx.doi.org/10.1364/OL.25.001768


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Abstract

We have developed a new coherent anti-Stokes Raman scattering (CARS) microscopy system with a collinear configuration for use in the fingerprint region. The system consists of a picosecond laser system and a transmission-type laser scanning microscope without a pinhole in front of the detector. The observable Raman-shift region is 900–1750 cm-1, the spectral resolution is 30cm-1, and the spatial resolution is smaller than 1 μm in the lateral direction and 3.2 μm in the depth direction, with objectives with a numerical aperture of 0.65. CARS spectra and images of polystyrene beads are demonstrated, and CARS imaging of a viable yeast cell is attempted.

© 2000 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(300.6450) Spectroscopy : Spectroscopy, Raman

Citation
Mamoru Hashimoto, Tsutomu Araki, and Satoshi Kawata, "Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration," Opt. Lett. 25, 1768-1770 (2000)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-25-24-1768


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