We demonstrate a new method for imaging through several millimeters of a turbid sample with a resolution of approximately 100 μm by combining aspects of confocal reflectance microscopy and diffuse optical tomography. By laterally displacing the pinhole aperture of a confocal microscope we can achieve small source–detector separations and detect minimally scattered light. A reconstruction algorithm based on the first Born approximation to the radiative transport equation is then used to reconstruct an image of a 100-μm absorbing object located 2 mm beneath the surface.
© 2000 Optical Society of America
[Optical Society of America ]
(170.0180) Medical optics and biotechnology : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
Andrew Dunn and David Boas, "Transport-based image reconstruction in turbid media with small source—detector separations," Opt. Lett. 25, 1777-1779 (2000)