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Optics Letters

Optics Letters


  • Vol. 25, Iss. 5 — Mar. 1, 2000
  • pp: 320–322

Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy

L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz  »View Author Affiliations

Optics Letters, Vol. 25, Issue 5, pp. 320-322 (2000)

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We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon-excited fluorescence (TPEF) can be used to rapidly image biological membranes labeled with a styryl dye. The SHG power is made compatible with the TPEF power by use of near-resonance excitation, in accord with a model based on the theory of phased-array antennas, which shows that the SHG radiation is highly structured. Because of its sensitivity to local asymmetry, SHG microscopy promises to be a powerful tool for the study of membrane dynamics.

© 2000 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes
(190.4350) Nonlinear optics : Nonlinear optics at surfaces
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials

L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, "Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy," Opt. Lett. 25, 320-322 (2000)

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