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Optics Letters

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  • Vol. 25, Iss. 5 — Mar. 1, 2000
  • pp: 320–322

Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy

L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz  »View Author Affiliations


Optics Letters, Vol. 25, Issue 5, pp. 320-322 (2000)
http://dx.doi.org/10.1364/OL.25.000320


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Abstract

We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon-excited fluorescence (TPEF) can be used to rapidly image biological membranes labeled with a styryl dye. The SHG power is made compatible with the TPEF power by use of near-resonance excitation, in accord with a model based on the theory of phased-array antennas, which shows that the SHG radiation is highly structured. Because of its sensitivity to local asymmetry, SHG microscopy promises to be a powerful tool for the study of membrane dynamics.

© 2000 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes
(190.4350) Nonlinear optics : Nonlinear optics at surfaces
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials

Citation
L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, "Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy," Opt. Lett. 25, 320-322 (2000)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-25-5-320


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References

  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  2. R. Gauderon, P. B. Lukins, and C. J. R. Sheppard, Opt. Lett. 23, 1209 (1998).
  3. Y. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, Opt. Lett. 22, 1323 (1997).
  4. P. J. Campagnola, M. Wei, and L. M. Loew, Biophys. J. 76, A95 (1999).
  5. G. Peleg, A. Lewis, M. Linial, and L. M. Loew, Proc. Natl. Acad. Sci. USA 96, 6700 (1999).
  6. S. R. Marder, D. N. Beratan, and L.-T. Cheng, Science 252, 103 (1991).
  7. T. Kogej, D. Beljonne, F. Meyers, J. W. Perry, S. R. Marder, and J. L. Brédas, Chem. Phys. Lett. 298, 1 (1998).
  8. O. Sandre, L. Moreaux, and F. Brochard, Proc. Natl. Acad. Sci. USA 96, 10, 588 (1999).
  9. L. M. Loew and L. L. Simpson, Biophys. J. 34, 353 (1981).
  10. D. S. Chemla and J. Zyss, eds., Nonlinear Optical Properties of Organic Molecules and Crystals (Academic, New York, 1984), Vol. 1.
  11. J. L. Oudar and D. S. Chemla, J. Chem. Phys. 66, 2664 (1977).
  12. Y. R. Shen, The Principles of Nonlinear Optics (Wiley, New York, 1984).

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