OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 26, Iss. 11 — Jun. 1, 2001
  • pp: 828–830

Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation

J. T. Frohn, H. F. Knapp, and A. Stemmer  »View Author Affiliations


Optics Letters, Vol. 26, Issue 11, pp. 828-830 (2001)
http://dx.doi.org/10.1364/OL.26.000828


View Full Text Article

Acrobat PDF (153 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A method for increasing lateral as well as axial resolution in fluorescence microscopy is presented. A passband with a high cutoff frequency throughout reciprocal space can be achieved by illumination of the object with spatially harmonic excitation patterns generated by the interference of two collimated laser beams. Theoretical calculations show an almost isotropic point-spread function with a FWHM near 100 nm.

© 2001 Optical Society of America

OCIS Codes
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(100.3020) Image processing : Image reconstruction-restoration
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

Citation
J. T. Frohn, H. F. Knapp, and A. Stemmer, "Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation," Opt. Lett. 26, 828-830 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-11-828

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited