A method for increasing lateral as well as axial resolution in fluorescence microscopy is presented. A passband with a high cutoff frequency throughout reciprocal space can be achieved by illumination of the object with spatially harmonic excitation patterns generated by the interference of two collimated laser beams. Theoretical calculations show an almost isotropic point-spread function with a FWHM near 100 nm.
© 2001 Optical Society of America
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(100.3020) Image processing : Image reconstruction-restoration
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
J. T. Frohn, H. F. Knapp, and A. Stemmer, "Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation," Opt. Lett. 26, 828-830 (2001)