We report a method for extracting the birefringence properties of biological samples with micrometer-scale resolution in three dimensions, using a new form of polarization-sensitive optical coherence tomography. The method measures net retardance, net fast axis, and total reflectivity as a function of depth into the sample. Polarization sensing is accomplished by illumination of the sample with at least three separate polarization states during consecutive acquisitions of the same pixel, A scan, or B scan. The method can be implemented by use of non-polarization-maintaining fiber and a single detector. In a calibration test of the system, net retardance was measured with an average error of 7.5° (standard deviation 2.2°) over the retardance range 0° to 180°, and a fast axis with average error of 4.8° over the range 0° to 180°.
© 2001 Optical Society of America
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(230.5440) Optical devices : Polarization-selective devices
Jonathan E. Roth, Jennifer A. Kozak, Siavash Yazdanfar, Andrew M. Rollins, and Joseph A. Izatt, "Simplified method for polarization-sensitive optical coherence tomography," Opt. Lett. 26, 1069-1071 (2001)