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Optics Letters

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  • Vol. 26, Iss. 16 — Aug. 15, 2001
  • pp: 1256–1258

Technique for detecting changes in fluorescence lifetime by means of optoelectronic circuit auto-oscillation

Michael J. O’Brien II, Emmanuil Rabinovich, S. R. J. Brueck, and Gabriel P. Lopez  »View Author Affiliations


Optics Letters, Vol. 26, Issue 16, pp. 1256-1258 (2001)
http://dx.doi.org/10.1364/OL.26.001256


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Abstract

We present a new, simple, inexpensive, and highly precise approach to excited-state fluorescence-lifetime-based measurements. The detection system consists of a closed-loop optoelectronic arrangement containing a radio frequency resonance amplifier, a fluorescence excitation light source, a fiber-optic delay line, and a photodetector. The system exhibits auto-oscillations in the form of intensity modulation. The oscillation frequency varies with the modulation phase shift of the fluorescent light. This frequency is used as the detection parameter, which is advantageous because frequency may be measured easily, inexpensively, and with high precision. This technique is well suited for chemical or biosensor applications.

© 2001 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(130.6010) Integrated optics : Sensors
(300.6380) Spectroscopy : Spectroscopy, modulation
(300.6500) Spectroscopy : Spectroscopy, time-resolved

Citation
Michael J. O’Brien II, Emmanuil Rabinovich, S. R. J. Brueck, and Gabriel P. Lopez, "Technique for detecting changes in fluorescence lifetime by means of optoelectronic circuit auto-oscillation," Opt. Lett. 26, 1256-1258 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-16-1256


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References

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