OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 26, Iss. 4 — Feb. 15, 2001
  • pp: 202–204

Resonant Raman detection of macular pigment levels in the living human retina

Igor V. Ermakov, Robert W. McClane, Werner Gellermann, and Paul S. Bernstein  »View Author Affiliations


Optics Letters, Vol. 26, Issue 4, pp. 202-204 (2001)
http://dx.doi.org/10.1364/OL.26.000202


View Full Text Article

Acrobat PDF (85 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We have used resonant Raman scattering as a novel, noninvasive in vivo optical technique to measure the concentration of macular carotenoid pigments in the living human retina. Using a backscattering geometry and resonant molecular excitation in the visible, we measure the Raman peaks that originate from the single- and double-bond stretch vibrations of the p -conjugated molecule's carbon backbone. The Raman signals scale linearly with carotenoid content, whereas the required laser excitation is well under safety limits for macular exposure. The Raman technique is objective and quantitative and may lead to a new method for rapid screening of carotenoid pigment levels in large human populations that are at risk for vision loss from age-related macular degeneration, the leading cause of blindness of the elderly in the United States.

© 2001 Optical Society of America

OCIS Codes
(160.4890) Materials : Organic materials
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4470) Medical optics and biotechnology : Ophthalmology
(290.5860) Scattering : Scattering, Raman
(300.6450) Spectroscopy : Spectroscopy, Raman
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment

Citation
Igor V. Ermakov, Robert W. McClane, Werner Gellermann, and Paul S. Bernstein, "Resonant Raman detection of macular pigment levels in the living human retina," Opt. Lett. 26, 202-204 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-4-202


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. R. A. Bone, J. T. Landrum, and S. L. Tarsis, Vision Res. 25, 1531 (1985).
  2. J. Handelman, D. M. Snodderly, A. J. Adler, M. D. Russett, and E. A. Dratz, Methods Enzymol. 213, 220 (1992).
  3. W. Schalch, P. Dayhaw-Barker, and F. M. Barker, in Nutritional and Environmental Influences on the Eye, A. Taylor, ed. (CRC, Boca Raton, Fla., 1999), p. 215.
  4. D. M. Snodderly, Am. J. Clin. Nutr. Suppl. 62, 1448S (1995).
  5. Eye Disease Case Control Study Group, Arch. Ophthalmol. 111, 104 (1993).
  6. J. M. Seddon, U. A. Ajani, R. D. Sperduto, R. Hiller, N. Blair, T. C. Burton, M. D. Farber, E. S. Gragourdas, J. Haller, D. T. Miller, L. A. Yannuzzi, and W. Willet, J. Am. Med. Assoc. 242, 1413 (1994).
  7. J. T. Landrum, R. A. Bone, and M. D. Kilburn, Adv. Pharmacol. 38, 537 (1997).
  8. B. R. Hammond, Jr., B. R. Wooten, and D. M. Snodderly, J. Opt. Soc. Am. A 14, 1187 (1997).
  9. W. Gellermann, M. D. Yoshida, R. W. McClane, N. Balashov, and P. S. Berstein, Proc. SPIE 3250, 8 (1998).
  10. P. S. Bernstein, M. D. Yoshida, N. B. Katz, R. W. McClane, and W. Gellermann, J. Vis. Sci. 39, 2003 (1998).
  11. A. P. Shreve, J. K. Trautman, T. G. Owens, and A. C. Albrecht, Phys. Lett. 178, 89 (1991).
  12. American National Standards Institute, American National Standard for Safe Use of Lasers, ANSI Z136.1–2000 (Laser Institute of America, Orlando, Fla., 2000), Sec. 8.3.
  13. Y. Koyama, I. Takatsuka, M. Nakata, and M. Tasumi, J. Raman Spectrosc. 19, 37 (1988).
  14. We achieved the background subtraction by fitting the fluorescence background with a fourth-order polynomial and subtracting it from the composite Raman–fluorescence spectra obtained for each measurement.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited