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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 26, Iss. 4 — Feb. 15, 2001
  • pp: 211–213

Rapid determination of the three-dimensional orientation of single molecules

John T. Fourkas  »View Author Affiliations


Optics Letters, Vol. 26, Issue 4, pp. 211-213 (2001)
http://dx.doi.org/10.1364/OL.26.000211


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Abstract

A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.

© 2001 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(260.5430) Physical optics : Polarization

Citation
John T. Fourkas, "Rapid determination of the three-dimensional orientation of single molecules," Opt. Lett. 26, 211-213 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-4-211

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