A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.
© 2001 Optical Society of America
John T. Fourkas, "Rapid determination of the three-dimensional orientation of single molecules," Opt. Lett. 26, 211-213 (2001)