OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 26, Iss. 4 — Feb. 15, 2001
  • pp: 211–213

Rapid determination of the three-dimensional orientation of single molecules

John T. Fourkas  »View Author Affiliations


Optics Letters, Vol. 26, Issue 4, pp. 211-213 (2001)
http://dx.doi.org/10.1364/OL.26.000211


View Full Text Article

Acrobat PDF (236 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.

© 2001 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(260.5430) Physical optics : Polarization

Citation
John T. Fourkas, "Rapid determination of the three-dimensional orientation of single molecules," Opt. Lett. 26, 211-213 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-4-211


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. T. Basché, W. E. Moerner, M. Orrit, and U. Wild, eds., Single Molecule Optical Detection, Imaging and Spectroscopy (Verlag-Chemie, Weinheim, Germany, 1997).
  2. W. E. Moerner and M. Orrit, Science 283, 1670 (1999).
  3. S. Weiss, Science 283, 1676 (1999).
  4. E. Betzig and R. J. Chichester, Science 262, 1422 (1993).
  5. D. A. Higgins, D. A. Vanden Bout, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
  6. T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
  7. A. G. T. Ruiter, J. A. Veerman, M. F. Garcia-Parajo, and N. F. van Hulst, J. Phys. Chem. A 101, 7318 (1997).
  8. T. Ha, J. Glass, Th. Enderle, D. S. Chemla, and S. Weiss, Phys. Rev. Lett. 80, 2093 (1998).
  9. R. M. Dickson, D. J. Norris, and W. E. Moerner, Phys. Rev. Lett. 81, 5322 (1998).
  10. A. P. Bartko and R. M. Dickson, J. Phys. Chem. B 103, 3053 (1999).
  11. A. P. Bartko and R. M. Dickson, J. Phys. Chem. B 103, 11237 (1999).
  12. T. Ha, T. A. Laurence, D. S. Chemla, and S. Weiss, J. Phys. Chem. B 103, 6839 (1999).
  13. D. Axelrod, Biophys. J. 26, 557 (1979).
  14. P. R. Callis, Annu. Rev. Phys. Chem. 48, 271 (1997).
  15. L. Novotny, E. J. Sanchez, and X. S. Xie, Ultramicroscopy 71, 21 (1998).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited