Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope
Optics Letters, Vol. 26, Issue 8, pp. 515-517 (2001)
http://dx.doi.org/10.1364/OL.26.000515
Acrobat PDF (521 KB)
Abstract
We describe the promising development of third-harmonic generation (THG) in laser scanning microscopy for study of the functional imaging of live biological cells. The dynamics of Ca2+ in biological cells is shown. The Ca2+ signal consists of a transient increase in the intracellular concentration. THG microscopy allows one to temporally visualize the release of Ca2+ from internal stores and (or) calcium influx.
© 2001 Optical Society of America
[Optical Society of America ]
OCIS Codes
(000.1430) General : Biology and medicine
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
Citation
L. Canioni, S. Rivet, L. Sarger, R. Barille, Pierre Vacher, and Pierre Voisin, "Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope," Opt. Lett. 26, 515-517 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-8-515
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Optical Contrast in Apertureless Microscopy (AO)
- Fluorescence lifetime of a molecule near a corrugated interface: application to near-field microscopy (JOSAA)
- Characterization of Near-Field Optical Probes (AO)
- Numerical Analysis of the Transmission Efficiency of Heat-Drawn and Chemically Etched Scanning Near-Field Optical Microscopes (AO)
- Photon Scanning-Tunneling Microscopy of Unstained Mammalian Cells and Chromosomes (AO)
Related Conference Papers
- Femtosecond High-Brightness Nanometer-Sized Coherent Light Source
- Fast-Scanning Two-Photon Fluorescence Imaging Using a Microelectromechanical Systems (MEMS) Two-Dimensional Scanning Mirror
- Arbitrary Focal-Field Polarization Control for Optical Microscopy
- Plasmon-Resonant Field Enhancement Metallic Tip-Substrate Systems
- Ultrastable Atomic Force Microscopy Using Laser-Based, Active Noise Cancellation
« Previous Article | Next Article »
OSA is a member of 