We describe the promising development of third-harmonic generation (THG) in laser scanning microscopy for study of the functional imaging of live biological cells. The dynamics of Ca<sup>2+</sup> in biological cells is shown. The Ca<sup>2+</sup> signal consists of a transient increase in the intracellular concentration. THG microscopy allows one to temporally visualize the release of Ca<sup>2+</sup> from internal stores and (or) calcium influx.
© 2001 Optical Society of America
L. Canioni, S. Rivet, L. Sarger, R. Barille, Pierre Vacher, and Pierre Voisin, "Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope," Opt. Lett. 26, 515-517 (2001)