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Optics Letters

Optics Letters


  • Vol. 26, Iss. 8 — Apr. 15, 2001
  • pp: 515–517

Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope

L. Canioni, S. Rivet, L. Sarger, R. Barille, Pierre Vacher, and Pierre Voisin  »View Author Affiliations

Optics Letters, Vol. 26, Issue 8, pp. 515-517 (2001)

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We describe the promising development of third-harmonic generation (THG) in laser scanning microscopy for study of the functional imaging of live biological cells. The dynamics of Ca2+ in biological cells is shown. The Ca2+ signal consists of a transient increase in the intracellular concentration. THG microscopy allows one to temporally visualize the release of Ca2+ from internal stores and (or) calcium influx.

© 2001 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy

L. Canioni, S. Rivet, L. Sarger, R. Barille, Pierre Vacher, and Pierre Voisin, "Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope," Opt. Lett. 26, 515-517 (2001)

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