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Optics Letters

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  • Vol. 27, Iss. 12 — Jun. 15, 2002
  • pp: 1031–1033

Compact two-photon fluorescence microscope based on a single-mode fiber coupler

Damian Bird and Min Gu  »View Author Affiliations


Optics Letters, Vol. 27, Issue 12, pp. 1031-1033 (2002)
http://dx.doi.org/10.1364/OL.27.001031


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Abstract

We present a two-photon fluorescence microscope based on a three-port single-mode optical fiber coupler. It is found that the coupler behaves as a low-pass filter that can deliver an ultrashort-pulsed laser beam of as much as 150 mW of power in the wavelength range from 770 to 870 nm as well as collect a two-photon fluorescence signal in the visible range. As a result of using the fiber coupler, the new two-photon imaging system exhibts a number of advantages, including a compact arrangement, freedom from vibration from lasers and electronic devices, self-alignment, reduction of multiple scattering, and an enhanced optical sectioning effect. The effectiveness of the new instrument is demonstrated with a set of three-dimensional images of biological samples. This instrument may make two-photon fluorescence endoscopy possible for in vivo medical applications.

© 2002 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.2350) Imaging systems : Fiber optics imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

Citation
Damian Bird and Min Gu, "Compact two-photon fluorescence microscope based on a single-mode fiber coupler," Opt. Lett. 27, 1031-1033 (2002)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-27-12-1031


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