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Optics Letters

Optics Letters


  • Vol. 27, Iss. 15 — Aug. 1, 2002
  • pp: 1324–1326

Second-harmonic-generation microscope with a microlens array scanner

Minoru Kobayashi, Katsumasa Fujita, Tomoyuki Kaneko, Tetsuro Takamatsu, Osamu Nakamura, and Satoshi Kawata  »View Author Affiliations

Optics Letters, Vol. 27, Issue 15, pp. 1324-1326 (2002)

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We propose a multifocus scanning technique in second-harmonic-generation (SHG) microscopy in which the SHG detection efficiency and the image acquisition rate are improved by several tens of times compared with typical single-focus scanning techniques. Because of a microlens array scanner, the laser beam of a mode-locked Ti:sapphire laser is split into ~100 beamlets, and the same number of foci are formed in the specimen. Parallel scanning of the foci enables us to observe real-time images of the specimen. The imaging properties and the usefulness of the microscope are demonstrated by SHG images of living HeLa cells and rat cardiac myocytes.

© 2002 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes
(190.4350) Nonlinear optics : Nonlinear optics at surfaces
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials

Minoru Kobayashi, Katsumasa Fujita, Tomoyuki Kaneko, Tetsuro Takamatsu, Osamu Nakamura, and Satoshi Kawata, "Second-harmonic-generation microscope with a microlens array scanner," Opt. Lett. 27, 1324-1326 (2002)

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