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Optics Letters

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  • Vol. 27, Iss. 22 — Nov. 15, 2002
  • pp: 2004–2006

Blood analysis by Raman spectroscopy

Annika M. K. Enejder, Tae-Woong Koo, Jeankun Oh, Martin Hunter, Slobodan Sasic, Michael S. Feld, and Gary L. Horowitz  »View Author Affiliations


Optics Letters, Vol. 27, Issue 22, pp. 2004-2006 (2002)
http://dx.doi.org/10.1364/OL.27.002004


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Abstract

Concentrations of multiple analytes were simultaneously measured in whole blood with clinical accuracy, without sample processing, using near-infrared Raman spectroscopy. Spectra were acquired with an instrument employing nonimaging optics, designed using Monte Carlo simulations of the influence of light-scattering–absorbing blood cells on the excitation and emission of Raman light in turbid medium. Raman spectra were collected from whole blood drawn from 31 individuals. Quantitative predictions of glucose, urea, total protein, albumin, triglycerides, hematocrit, and hemoglobin were made by means of partial least-squares (PLS) analysis with clinically relevant precision (r2 values >0.93). The similarity of the features of the PLS calibration spectra to those of the respective analyte spectra illustrates that the predictions are based on molecular information carried by the Raman light. This demonstrates the feasibility of using Raman spectroscopy for quantitative measurements of biomolecular contents in highly light-scattering and absorbing media.

© 2002 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1580) Medical optics and biotechnology : Chemometrics
(170.1610) Medical optics and biotechnology : Clinical applications
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(170.7050) Medical optics and biotechnology : Turbid media

Citation
Annika M. K. Enejder, Tae-Woong Koo, Jeankun Oh, Martin Hunter, Slobodan Sasic, Michael S. Feld, and Gary L. Horowitz, "Blood analysis by Raman spectroscopy," Opt. Lett. 27, 2004-2006 (2002)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-27-22-2004


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