OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 27, Iss. 4 — Feb. 15, 2002
  • pp: 249–251

Near-infrared Raman spectroscopy of single optically trapped biological cells

Changan Xie, Mumtaz A. Dinno, and Yong-qing Li  »View Author Affiliations


Optics Letters, Vol. 27, Issue 4, pp. 249-251 (2002)
http://dx.doi.org/10.1364/OL.27.000249


View Full Text Article

Acrobat PDF (321 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report on the development and testing of a compact laser tweezers Raman spectroscopy (LTRS) system. The system combines optical trapping and near-infrared Raman spectroscopy for manipulation and identification of single biological cells in solution. A low-power diode laser at 785 nm was used for both trapping and excitation for Raman spectroscopy of the suspended microscopic particles. The design of the LTRS system provides high sensitivity and permits real-time spectroscopic measurements of the biological sample. The system was calibrated by use of polystyrene microbeads and tested on living blood cells and on both living and dead yeast cells. As expected, different images and Raman spectra were observed for the different cells. The LTRS system may provide a valuable tool for the study of fundamental cellular processes and the diagnosis of cellular disorders.

© 2002 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy

Citation
Changan Xie, Mumtaz A. Dinno, and Yong-qing Li, "Near-infrared Raman spectroscopy of single optically trapped biological cells," Opt. Lett. 27, 249-251 (2002)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-27-4-249


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, Opt. Lett. 11, 288 (1986).
  2. A. Ashkin, K. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
  3. K. Svoboda and S. M. Block, Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
  4. M. P. Sheetz, ed., Laser Tweezers in Cell Biology, Vol. 55 of Methods in Cell Biology (Academic, San Diego, Calif., 1998).
  5. J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, Phys. Rev. Lett. 84, 5451 (2000).
  6. K. Visscher, M. J. Schnitzer, and S. M. Block, Nature 400, 184 (1999).
  7. K. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2656 (1999).
  8. K. Konig, H. Liang, M. W. Berns, and B. J. Tromberg, Nature 377, 20 (1995).
  9. N. Kikatani, H.-B. Kim, S. Habuchi, and M. Chiba, Trends Anal. Chem. 18, 675 (1999).
  10. Y. Liu, G. J. Sonek, M. W. Berns, K. Konig, and B. J. Tromberg, Opt. Lett. 20, 2246 (1995).
  11. M. Lankers, J. Popp, and W. Kiefer, Appl. Spectrosc. 48, 1166 (1994).
  12. K. Ajito, Appl. Spectrosc. 52, 339 (1998).
  13. K. Ajito and K. Torimitsu, Trends Anal. Chem. 20, 255 (2001).
  14. S. R. Hawi, W. B. Campbell, A. Kajdacsy-Balla, R. Murphy, F. Adar, and K. Nithipatikom, Cancer Lett. 110, 35 (1996).
  15. Y. Q. Li, W. H. Burkett, and M. Xiao, Opt. Lett. 21, 982 (1996).
  16. B. R. Wood, B. Tait, and D. McNaughton, Biochim. Biophys. Acta 1539, 58 (2001).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited