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Optics Letters

Optics Letters


  • Vol. 27, Iss. 4 — Feb. 15, 2002
  • pp: 264–266

Imaging microscopic viscosity with confocal scanning optical tweezers

Boaz A. Nemet, Yossef Shabtai, and Mark Cronin-Golomb  »View Author Affiliations

Optics Letters, Vol. 27, Issue 4, pp. 264-266 (2002)

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The techniques of confocal microscopy and optical tweezers have shown themselves to be powerful tools in biological and medical research. We combine these methods to develop a minimally invasive instrument that is capable of making hydrodynamic measurements more rapidly than is possible with other devices. This result leads to the possibility of making scanning images of the viscosity distribution of materials around biopolymer-producing cells. 100 × 100 images can be taken with 0.5-µm spatial resolution in 3 min. An image of the viscosity distribution around a pullulan-producing cell of Aureobasidium pullulans is shown as an example.

© 2002 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy

Boaz A. Nemet, Yossef Shabtai, and Mark Cronin-Golomb, "Imaging microscopic viscosity with confocal scanning optical tweezers," Opt. Lett. 27, 264-266 (2002)

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