OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 27, Iss. 4 — Feb. 15, 2002
  • pp: 264–266

Imaging microscopic viscosity with confocal scanning optical tweezers

Boaz A. Nemet, Yossef Shabtai, and Mark Cronin-Golomb  »View Author Affiliations


Optics Letters, Vol. 27, Issue 4, pp. 264-266 (2002)
http://dx.doi.org/10.1364/OL.27.000264


View Full Text Article

Acrobat PDF (124 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The techniques of confocal microscopy and optical tweezers have shown themselves to be powerful tools in biological and medical research. We combine these methods to develop a minimally invasive instrument that is capable of making hydrodynamic measurements more rapidly than is possible with other devices. This result leads to the possibility of making scanning images of the viscosity distribution of materials around biopolymer-producing cells. 100 × 100 images can be taken with 0.5-µm spatial resolution in 3 min. An image of the viscosity distribution around a pullulan-producing cell of Aureobasidium pullulans is shown as an example.

© 2002 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy

Citation
Boaz A. Nemet, Yossef Shabtai, and Mark Cronin-Golomb, "Imaging microscopic viscosity with confocal scanning optical tweezers," Opt. Lett. 27, 264-266 (2002)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-27-4-264


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. A. Pralle, E. L. Florin, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S71 (1998).
  2. A. Meller, R. Bar-Ziv, T. Tlusty, E. Moses, J. Stavans, and S. A. Safran, Biophys. J. 74, 1541 (1998).
  3. M. T. Valentine, L. E. Dewalt, and H. D. OuYang, J. Phys. Condens. Matter 8, 9477 (1996).
  4. B. Nemet and M. Cronin-Golumb are preparing a manuscript to be called “Measuring microscopic viscosity with optical tweezers as a confocal probe.”
  5. Some axial offset is required because the probe microsphere is not trapped at exactly the focal point of the laser in the sample.
  6. M. Doi, Introduction to Polymer Physics (Oxford U. Press, Oxford, England, 1996).
  7. Y. Shabtai and I. Mukmenev, Appl. Microbiol. Biotechnol. 43, 595 (1995).
  8. L. Malmqvist and H. M. Hertz, Opt. Lett. 19, 853 (1994).
  9. M. E. J. Friese, A. G. Truscott, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Appl. Opt. 38, 6597 (1999).
  10. T. G. Mason and D. A. Weitz, Phys. Rev. Lett. 74, 7 (1995).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited