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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 28, Iss. 11 — Jun. 1, 2003
  • pp: 902–904

Multiphoton endoscopy

Juergen C. Jung and Mark J. Schnitzer  »View Author Affiliations


Optics Letters, Vol. 28, Issue 11, pp. 902-904 (2003)
http://dx.doi.org/10.1364/OL.28.000902


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Abstract

Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350–1000μm in diameter) based on gradient-index microlenses that effectively eliminate self-phase modulation within the endoscope. Laser-scanning multiphoton fluorescence endoscopy exhibits micrometer-scale resolution. We used multiphoton endoscopes to image fluorescently labeled neurons and dendrites.

© 2003 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy

Citation
Juergen C. Jung and Mark J. Schnitzer, "Multiphoton endoscopy," Opt. Lett. 28, 902-904 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-11-902


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