Recently Török and colleagues published in a series of papers a general wave-optical approach to calculating the light-collection efficiency function (CEF) for confocal imaging of dipole emitters [J. Mod. Opt. 45, 1681 (1998); J. Microsc. 194, 127 (1999); Opt. Lett. 25, 1463 (2000)]. In their theory they did not address the possibility that the lifetimes of fluorescing molecules can change significantly near interfaces, which has a direct effect on the CEF. The research of Török and colleagues is extended here to include this effect, which may become important for imaging near surfaces.
© 2003 Optical Society of America
[Optical Society of America ]
(110.4850) Imaging systems : Optical transfer functions
(180.1790) Microscopy : Confocal microscopy
(220.1010) Optical design and fabrication : Aberrations (global)
(300.2530) Spectroscopy : Fluorescence, laser-induced
Jörg Enderlein and Martin Bhmer, "Influence of interface dipole interactions on the efficiency of fluorescence light collection near surfaces," Opt. Lett. 28, 941-943 (2003)