OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 28, Iss. 14 — Jul. 15, 2003
  • pp: 1221–1223

Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells

Andrew C. Millard, Lei Jin, Aaron Lewis, and Leslie M. Loew  »View Author Affiliations


Optics Letters, Vol. 28, Issue 14, pp. 1221-1223 (2003)
http://dx.doi.org/10.1364/OL.28.001221


View Full Text Article

Acrobat PDF (277 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report what is to our knowledge the first optical imaging of voltage-clamped cells by second-harmonic generation. For the membrane-staining styryl dye di-4-ANEPPS, we determined the sensitivity of second-harmonic generation to be 18%/100 mV at an excitation wavelength of 850 nm. This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells.

© 2003 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4350) Nonlinear optics : Nonlinear optics at surfaces
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials

Citation
Andrew C. Millard, Lei Jin, Aaron Lewis, and Leslie M. Loew, "Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells," Opt. Lett. 28, 1221-1223 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-14-1221

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited