Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells
Optics Letters, Vol. 28, Issue 14, pp. 1221-1223 (2003)
http://dx.doi.org/10.1364/OL.28.001221
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Abstract
We report what is to our knowledge the first optical imaging of voltage-clamped cells by second-harmonic generation. For the membrane-staining styryl dye di-4-ANEPPS, we determined the sensitivity of second-harmonic generation to be 18%/100 mV at an excitation wavelength of 850 nm. This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells.
© 2003 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4350) Nonlinear optics : Nonlinear optics at surfaces
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
Citation
Andrew C. Millard, Lei Jin, Aaron Lewis, and Leslie M. Loew, "Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells," Opt. Lett. 28, 1221-1223 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-14-1221
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