OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 28, Iss. 17 — Sep. 1, 2003
  • pp: 1552–1554

Two-photon fluorescence endoscopy with a micro-optic scanning head

Damian Bird and Min Gu  »View Author Affiliations

Optics Letters, Vol. 28, Issue 17, pp. 1552-1554 (2003)

View Full Text Article

Acrobat PDF (760 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A major obstacle in the race to develop two-photon fluorescence endoscopy is the use of complicated bulk optics to transmit an ultrashort-pulsed laser beam and return the emitted fluorescence signal. We describe an all-fiber two-photon fluorescence microendoscope based on a single-mode optical fiber coupler, a microprism, and a gradient-index rod lens. It is found that the new endoscope exhibits an axial resolution of 3.2 μm and is capable of imaging transverse cross sections of internal cylindrical structures as small as approximately 3.0 mm in diameter. This device demonstrates the potential for developing a real-time diagnostic tool for biomedical research without the need for surgical biopsy and may find applications in photodynamic therapy, microsurgery, and early cancer detection.

© 2003 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

Damian Bird and Min Gu, "Two-photon fluorescence endoscopy with a micro-optic scanning head," Opt. Lett. 28, 1552-1554 (2003)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1999).
  2. G. A. Wagnieres, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998).
  3. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  4. T. Dabbs and M. Glass, Appl. Opt. 31, 705 (1992).
  5. J. Benschop and G. van Rosmalen, Appl. Opt. 30, 1179 (1991).
  6. S. Kimura and T. Wilson, Appl. Opt. 30, 2143 (1991).
  7. M. Gu, C. J. R. Sheppard, and X. Gan, J. Opt. Soc. Am. A 8, 1755 (1991).
  8. D. Bird and M. Gu, Opt. Lett. 27, 1031 (2002).
  9. G. J. Tearney, S. A. Boppart, B. E. Bouma, M. E. Brezinski, N. J. Weissman, J. F. Southern, and J. G. Fujimoto, Opt. Lett. 21, 543 (1996).
  10. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (World Scientific, Singapore, 1996).
  11. D. Bird and M. Gu, Appl. Opt. 41, 1852 (2002).
  12. G. P. Agrawal, Nonlinear Fiber Optics (Academic, San Diego, Calif., 1989).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited