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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 28, Iss. 18 — Sep. 15, 2003
  • pp: 1654–1656

Multiple imaging axis microscopy improves resolution for thick-sample applications

Jim Swoger, Jan Huisken, and Ernst H. K. Stelzer  »View Author Affiliations


Optics Letters, Vol. 28, Issue 18, pp. 1654-1656 (2003)
http://dx.doi.org/10.1364/OL.28.001654


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Abstract

The multiple imaging axis microscope (MIAM) is a wide-field optical microscope that observes a sample simultaneously from multiple directions without requiring the sample to be rotated or tilted. The prototype is capable of high-resolution imaging of the interior of a 300-μm -diameter sample consisting of fluorescent microbeads suspended in an agarose gel. Compared with a single-axis system, the MIAM can achieve a reduction of the axial point-spread function elongation by a factor of 5.8 and a 3.5-fold improvement in volume resolution by simple linear image combination techniques.

© 2003 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
Jim Swoger, Jan Huisken, and Ernst H. K. Stelzer, "Multiple imaging axis microscopy improves resolution for thick-sample applications," Opt. Lett. 28, 1654-1656 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-18-1654


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References

  1. S. Grill and E. H. K. Stelzer, J. Opt. Soc. Am. A 16, 2658 (1999).
  2. E. H. K. Stelzer, J. Microsc. 189, 15 (1998).
  3. S. Hell and E. H. K. Stelzer, J. Opt. Soc. Am. A 9, 2159 (1992).
  4. E. H. K. Stelzer and S. Lindek, Opt. Commun. 111, 536 (1994).
  5. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 4951 (1990).
  6. P. J. Shaw, D. A. Agard, Y. Hiraoka, and J. W. Sedat, Biophys. J. 55, 101 (1989).
  7. K. Sätzler and R. Elis, Bioimaging 5, 171 (1997).
  8. J. Bradl, M. Hausmann, B. Schneider, B. Rinke, and C. Cremer, J. Microsc. 175, 211 (1994).
  9. S. Kikuchi, K. Sonobe, L. S. Didharta, and N. Ohyama, Opt. Commun. 107, 432 (1994).
  10. S. Kikuchi, K. Sonobe, S. Mashiko, Y. Hiraoka, and N. Ohyama, Opt. Commun. 138, 21 (1997).
  11. K. G. Larkin, M. A. Oldfield, and H. Klemm, Opt. Commun. 139, 99 (1997).
  12. L. Gottesfeld Brown, ACM Comput. Surv. 24, 325 (1992).
  13. P. J. Verveer, M. J. Gemkow, and T. M. Jovin, J. Microsc. 193, 50 (1999).

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