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Optics Letters

Optics Letters

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  • Vol. 28, Iss. 19 — Oct. 1, 2003
  • pp: 1742–1744

Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

G. McConnell, G. L. Smith, J. M. Girkin, A. M. Gurney, and A. I. Ferguson  »View Author Affiliations


Optics Letters, Vol. 28, Issue 19, pp. 1742-1744 (2003)
http://dx.doi.org/10.1364/OL.28.001742


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Abstract

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2.

© 2003 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(190.7110) Nonlinear optics : Ultrafast nonlinear optics

Citation
G. McConnell, G. L. Smith, J. M. Girkin, A. M. Gurney, and A. I. Ferguson, "Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source," Opt. Lett. 28, 1742-1744 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-19-1742


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