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Optics Letters

Optics Letters


  • Vol. 28, Iss. 19 — Oct. 1, 2003
  • pp: 1772–1774

Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy

Chau-Hwang Lee, Hui-Yu Chiang, and Hong-Yao Mong  »View Author Affiliations

Optics Letters, Vol. 28, Issue 19, pp. 1772-1774 (2003)

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Using the nanometer depth sensitivity of differential confocal microscopy, we detect surface features of lateral dimensions smaller than the diffraction limit without fluorescence labeling. The lateral resolution of the topographic images is further enhanced by a maximum-likelihood estimation algorithm. Based on the comparison of signal and noise at high spatial frequency, we estimate the best lateral resolution of the enhanced images to be 0.15λ . In addition, on composite samples this technique can simultaneously display sub-diffraction-limit topographic features and reflectivity heterogeneity.

© 2003 Optical Society of America

OCIS Codes
(100.3020) Image processing : Image reconstruction-restoration
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy

Chau-Hwang Lee, Hui-Yu Chiang, and Hong-Yao Mong, "Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy," Opt. Lett. 28, 1772-1774 (2003)

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