Experimental verification of the ability to alter the sensitivity to fluorophore layers in turbid media by varying illumination-collection geometry is presented. Fiber-optic probes and two-layer, fluorophore-doped, turbid phantoms are used to elucidate the roles of spot size, illumination–collection fiber separation, and probe–sample spacing. Variations in single- and multiple-fiber probe design parameters produce significant changes in the relative sensitivity to sample layers in a manner that agrees with prior computational studies.
© 2003 Optical Society of America
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
T. Joshua Pfefer, L. Stephanie Matchette, Amanda M. Ross, and Marwood N. Ediger, "Selective detection of fluorophore layers in turbid media: the role of fiber-optic probe design," Opt. Lett. 28, 120-122 (2003)