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Optics Letters

Optics Letters


  • Vol. 28, Iss. 2 — Jan. 15, 2003
  • pp: 69–71

Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging

Michael Speidel, Alexandr Jonáš, and Ernst-Ludwig Florin  »View Author Affiliations

Optics Letters, Vol. 28, Issue 2, pp. 69-71 (2003)

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We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40μmχ60χm χ3m . With the technique presented, the structure–mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.

© 2003 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

Michael Speidel, Alexandr Jonáš, and Ernst-Ludwig Florin, "Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging," Opt. Lett. 28, 69-71 (2003)

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  1. M. De Brabander, G. Geuens, R. Nuydens, M. Moeremans, and J. De Mey, Cytobios 43, 273 (1985).
  2. M. J. Saxton and K. Jacobson, Annu. Rev. Biophys. Biomol. Struct. 26, 373 (1997).
  3. J. C. Crocker and D. G. Grier, J. Colloid. Interf. Sci. 179, 298 (1996).
  4. R. Yasuda, H. Noji, M. Yoshida, K. Kinosita, Jr., and H. Itoh, Nature 410, 898 (2001).
  5. D. H. Burns, J. B. Callis, G. D. Christian, and E. R. Davidson, Appl. Opt. 24, 154 (1985).
  6. H. P. Kao and A. S. Verkman, Biophys. J. 67, 1291 (1994).
  7. M. Schmidt, M. Nagorni, and S. W. Hell, Rev. Sci. Instrum. 71, 2742 (2000).
  8. C. M. Ajo-Franklin, L. Kam, and S. G. Boxer, Proc. Natl. Acad. Sci. USA 98, 13643 (2001).
  9. S. F. Gibson and F. Lanni, J. Opt. Soc. Am. A 6, 1357 (1989).
  10. D. A. Agard, Y. Hiraoka, P. Shaw, and J. W. Sedat, in Fluorescence Microscopy of Living Cells in Culture, Part B, D. L. Taylor and Y. Wang, eds., Vol. 30 of Methods in Cell Biology (Academic, San Diego, Calif., 1989), pp. 353–377.
  11. S. Hell, G. Reiner, C. Cremer, and E. H. K. Stelzer, J. Microsc. (Oxford) 169, 391 (1993).
  12. A. Pralle and E.-L. Florin, in Atomic Force Microscopy in Cell Biology, B. P. Jena and J. K. H. Hörber, eds., Vol. 68 of Methods in Cell Biology (Academic, San Diego, Calif., 2002), pp. 193–213.
  13. N. Bobroff, Rev. Sci. Instrum. 57, 1152 (1986).
  14. C. Tischer, S. Altmann, S. Fisinger, E. H. K. Stelzer, J. K. H. Hörber, and E.-L. Florin, Appl. Phys. Lett. 79, 3878 (2001).

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