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Optics Letters

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  • Vol. 28, Iss. 2 — Jan. 15, 2003
  • pp: 69–71

Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging

Michael Speidel, Alexandr Jonáš, and Ernst-Ludwig Florin  »View Author Affiliations


Optics Letters, Vol. 28, Issue 2, pp. 69-71 (2003)
http://dx.doi.org/10.1364/OL.28.000069


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Abstract

We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40μmχ60χm χ3m . With the technique presented, the structure–mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.

© 2003 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

Citation
Michael Speidel, Alexandr Jonáš, and Ernst-Ludwig Florin, "Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging," Opt. Lett. 28, 69-71 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-2-69


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