OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 28, Iss. 21 — Nov. 1, 2003
  • pp: 2070–2072

Metabolism-enhanced tumor localization by fluorescence imaging: in vivo animal studies

Y. Chen, G. Zheng, Z. H. Zhang, D. Blessington, M. Zhang, H. Li, Q. Liu, L. Zhou, X. Intes, S. Achilefu, and B. Chance  »View Author Affiliations

Optics Letters, Vol. 28, Issue 21, pp. 2070-2072 (2003)

View Full Text Article

Acrobat PDF (330 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a high-sensitivity near-infrared optical imaging system for noninvasive cancer detection and localization based on molecularly labeled fluorescent contrast agents. This frequency-domain system utilizes the interferencelike pattern of diffuse photon density waves to achieve high detection sensitivity and localization accuracy for the fluorescent heterogeneity embedded inside the scattering media. A two-dimensional localization map is obtained through reflectance probe geometry and goniometric reconstruction. In vivo measurements with a tumor-bearing mouse model by use of the novel Cypate-mono-2-deoxy-glucose fluorescent contrast agent, which targets the enhanced tumor glycolysis, demonstrate the feasibility of detection of a 2-cm-deep subsurface tumor in the tissuelike medium, with a localization accuracy within 2–3 mm.

© 2003 Optical Society of America

OCIS Codes
(110.5100) Imaging systems : Phased-array imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.5270) Medical optics and biotechnology : Photon density waves
(300.2530) Spectroscopy : Fluorescence, laser-induced

Y. Chen, G. Zheng, Z. H. Zhang, D. Blessington, M. Zhang, H. Li, Q. Liu, L. Zhou, X. Intes, S. Achilefu, and B. Chance, "Metabolism-enhanced tumor localization by fluorescence imaging: in vivo animal studies," Opt. Lett. 28, 2070-2072 (2003)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. V. Ntziachristos, A. G. Yodh, M. Schnall, and B. Chance, Proc. Natl. Acad. Sci. USA 97, 2767 (2000).
  2. T. O. McBride, B. W. Pogue, S. D. Jiang, U. L. Osterberg, K. D. Paulsen, and S. P. Poplack, Opt. Lett. 26, 822 (2001).
  3. X. Intes, J. Ripoll, Y. Chen, S. Nioka, A. G. Yodh, and B. Chance, Med. Phys. 30, 1039 (2003).
  4. R. Weissleder and U. Mahmood, Radiology 219, 316 (2001).
  5. S. Achilefu, R. B. Dorshow, J. E. Bugaj, and R. Rajagopalan, Invest. Radiol. 35, 479 (2000).
  6. M. E. Phelps, Proc. Natl. Acad. Sci. USA 97, 9226 (2000).
  7. M. Zhang, Z. H. Zhang, D. Blessington, H. Li, T. M. Busch, V. Madrak, J. Miles, J. D. Glickson, and G. Zheng, Bioconjugate Chem. 14, 709 (2003).
  8. V. Ntziachristos, C. H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
  9. M. J. Eppstein, D. J. Hawrysz, A. Godavarty, and E. M. Sevick-Muraca, Proc. Natl. Acad. Sci. USA 99, 9619 (2002).
  10. A. Eidsath, V. Chernomordik, A. Gandjbakhche, P. Smith, and A. Russo, Phys. Med. Biol. 47, 4079 (2002).
  11. J. M. Schmitt, A. Knuttel, and J. R. Knutson, J. Opt. Soc. Am. A 9, 1832 (1992).
  12. B. Chance, K. Kang, L. He, J. Weng, and E. Sevick, Proc. Natl. Acad. Sci. USA 90, 3423 (1993).
  13. M. A. O’Leary, D. A. Boas, X. D. Li, B. Chance, and A. G. Yodh, Opt. Lett. 21, 158 (1996).
  14. X. Intes, Y. Chen, X. Li, and B. Chance, Appl. Opt. 41, 3999 (2002).
  15. Y. Chen, C. P. Mu, X. Intes, D. Blessington, and B. Chance, Rev. Sci. Instrum. 74, 3466 (2003).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited