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Optics Letters

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  • Vol. 28, Iss. 23 — Dec. 1, 2003
  • pp: 2315–2317

Depth-resolved multiphoton polarization microscopy by third-harmonic generation

Dan Oron, Eran Tal, and Yaron Silberberg  »View Author Affiliations


Optics Letters, Vol. 28, Issue 23, pp. 2315-2317 (2003)
http://dx.doi.org/10.1364/OL.28.002315


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Abstract

We achieve depth-resolved polarization microscopy by measuring third-harmonic generation induced by a tightly focused circularly polarized beam. In crystals exhibiting strong birefringence this signal is dominated by positively phase-matched third-harmonic generation. This process occurs in only optically anisotropic media, in which the birefringence compensates for the phase mismatch between the fundamental and the third harmonic induced by dispersion. Both the intensity and the polarization of the emitted signal provide information on the local optical anisotropy. We demonstrate the technique by imaging biogenic crystals in sea urchin larval spicules.

© 2003 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.7110) Nonlinear optics : Ultrafast nonlinear optics

Citation
Dan Oron, Eran Tal, and Yaron Silberberg, "Depth-resolved multiphoton polarization microscopy by third-harmonic generation," Opt. Lett. 28, 2315-2317 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-23-2315


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References

  1. W. Denk, J. H. Stricker, and W. W. Webb, Science 248, 73 (1990).
  2. S. Maiti, J. B. Shear, R. M. Williams, W. R. Zipfel, and W. W. Webb, Science 275, 530 (1997).
  3. G. Peleg, A. Lewis, O. Bouevitch, L. Loew, D. Parnas, and M. Linial, Bioimaging 4, 215 (1996).
  4. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
  5. M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, J. Microsc. 191, 266 (1998).
  6. J. A. Squier, M. Muller, G. J. Brakenhoff, and K. R. Wilson, Opt. Express 3, 315 (1998), http://www.opticsexpress.org.
  7. D. Yelin and Y. Silberberg, Opt. Express 5, 169 (1999), http://www.opticsexpress.org.
  8. D. Yelin and Y. Silberberg, Miscrosc. Anal. 80, 15 (2000).
  9. A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
  10. J. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, Biophys. J. 83, 502 (2002).
  11. E. O. Potma, W. P. de Boeij, P. J. M. van Haastert, and D. A. Wiersma, Proc. Natl. Acad. Sci. USA 98, 1577 (2001).
  12. N. Dudovich, D. Oron, and Y. Silberberg, Nature 418, 512 (2002).
  13. R. Oldenburg, Nature 381, 811 (1996).
  14. A. M. Glazer, J. G. Lewis, and W. Kaminsky, Proc. R. Soc. London Ser. A 452, 2751 (1996), and references therein.
  15. J. R. Kuhn, Z. Wu, and M. Poenie, Biophys. J. 80, 972 (2001).
  16. R. Boyd, Nonlinear Optics (Academic, New York, 1992).
  17. M. D. Levenson, Introduction to Nonlinear Laser Spectroscopy (Academic, New York, 1982).
  18. V. V. Yakovlev and S. V. Govorkov, Appl. Phys. Lett. 79, 4136 (2001).
  19. E. Beniash, J. Aizenberg, L. Addadi, and S. Weiner, Proc. R. Soc. London Ser. B 264, 461 (1997).
  20. A. Penzkofer, F. Ossig, and P. Qiu, Appl. Phys. B 47, 71 (1988).
  21. Note that, because of the large positive phase mismatch in calcite, the THG signal as derived in relation 2 becomes exceedingly small when objectives with a small N.A. are used. We have verified this experimentally by using 0.25-N.A. and 0.57-N.A. objectives.
  22. D. Yelin, D. Oron, E. Korokotian, M. Segal, and Y. Silberberg, Appl. Phys. B 74, S97 (2002).
  23. B. R. Masters, ed., Selected Papers on Multiphoton Excitation Microscopy (SPIE, Bellingham, Washington, 2003).

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