Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200–1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.
© 2003 Optical Society of America
(170.1870) Medical optics and biotechnology : Dermatology
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
Chi-Kuang Sun, Cheng-Chi Chen, Shi-Wei Chu, Tsung-Han Tsai, Yung-Chih Chen, and Bai-Ling Lin, "Multiharmonic-generation biopsy of skin," Opt. Lett. 28, 2488-2490 (2003)