We describe a simple holographic method that has enabled us to capture as a single data set the trajectories of micrometer-sized objects suspended in water. By subtracting consecutive holograms of a particle suspension and then adding these difference holograms, we constructed a final data set that contains the time evolution of the particle trajectories free from spurious background interference effects. The method is illustrated by a recording of the motion of 5–10-μm diameter algae in water.
© 2003 Optical Society of America
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(100.2000) Image processing : Digital image processing
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
W. Xu, M. H. Jericho, H. J. Kreuzer, and I. A. Meinertzhagen, "Tracking particles in four dimensions with in-line holographic microscopy," Opt. Lett. 28, 164-166 (2003)