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Optics Letters

Optics Letters


  • Vol. 28, Iss. 3 — Feb. 1, 2003
  • pp: 164–166

Tracking particles in four dimensions with in-line holographic microscopy

W. Xu, M. H. Jericho, H. J. Kreuzer, and I. A. Meinertzhagen  »View Author Affiliations

Optics Letters, Vol. 28, Issue 3, pp. 164-166 (2003)

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We describe a simple holographic method that has enabled us to capture as a single data set the trajectories of micrometer-sized objects suspended in water. By subtracting consecutive holograms of a particle suspension and then adding these difference holograms, we constructed a final data set that contains the time evolution of the particle trajectories free from spurious background interference effects. The method is illustrated by a recording of the motion of 5–10-μm diameter algae in water.

© 2003 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(100.2000) Image processing : Digital image processing
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

W. Xu, M. H. Jericho, H. J. Kreuzer, and I. A. Meinertzhagen, "Tracking particles in four dimensions with in-line holographic microscopy," Opt. Lett. 28, 164-166 (2003)

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