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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 28, Iss. 4 — Feb. 15, 2003
  • pp: 224–226

Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection

C. Yang and J. Mertz  »View Author Affiliations


Optics Letters, Vol. 28, Issue 4, pp. 224-226 (2003)
http://dx.doi.org/10.1364/OL.28.000224


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Abstract

We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for descanning.

© 2003 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

Citation
C. Yang and J. Mertz, "Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection," Opt. Lett. 28, 224-226 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-4-224

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