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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 28, Iss. 4 — Feb. 15, 2003
  • pp: 224–226

Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection

C. Yang and J. Mertz  »View Author Affiliations


Optics Letters, Vol. 28, Issue 4, pp. 224-226 (2003)
http://dx.doi.org/10.1364/OL.28.000224


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Abstract

We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for descanning.

© 2003 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

Citation
C. Yang and J. Mertz, "Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection," Opt. Lett. 28, 224-226 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-4-224


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References

  1. M. Minsky, “Microscopy Apparatus,” U.S. patent 3, 013, 467 (November 7, 1957).
  2. T. Wilson and C. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, London, 1984).
  3. A. E. Dixon, S. Damaskinos, and M. R. Atkinson, Nature 351, 551 (1991).
  4. G. J. Brakenhoff, J. Microsc. 117, 233 (1979).
  5. M. Born and E. Wolf, Principles of Optics, 7th ed. (Cambridge U. Press, Cambridge, 1999).
  6. C. Bohren and D. Huffman, Absorption and Scattering of Light by Small Particles (Wiley-Interscience, New York, 1983).
  7. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).

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