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Optics Letters

Optics Letters


  • Vol. 28, Iss. 4 — Feb. 15, 2003
  • pp: 224–226

Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection

C. Yang and J. Mertz  »View Author Affiliations

Optics Letters, Vol. 28, Issue 4, pp. 224-226 (2003)

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We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for descanning.

© 2003 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

C. Yang and J. Mertz, "Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection," Opt. Lett. 28, 224-226 (2003)

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