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Optics Letters

Optics Letters


  • Vol. 28, Iss. 4 — Feb. 15, 2003
  • pp: 272–274

Shift of whispering-gallery modes in microspheres by protein adsorption

S. Arnold, M. Khoshsima, I. Teraoka, S. Holler, and F. Vollmer  »View Author Affiliations

Optics Letters, Vol. 28, Issue 4, pp. 272-274 (2003)

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Biosensors based on the shift of whispering-gallery modes in microspheres accompanying protein adsorption are described by use of a perturbation theory. For random spatial adsorption, theory predicts that the shift should be inversely proportional to microsphere radius R and proportional to protein surface density and excess polarizability. Measurements are found to be consistent with the theory, and the correspondence enables the average surface area occupied by a single protein to be estimated. These results are consistent with crystallographic data for bovine serum albumin. The theoretical shift for adsorption of a single protein is found to be extremely sensitive to the target region, with adsorption in the most sensitive region varying as 1/R5/2 . Specific parameters for single protein or virus particle detection are predicted.

© 2003 Optical Society of America

OCIS Codes
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(300.6490) Spectroscopy : Spectroscopy, surface

S. Arnold, M. Khoshsima, I. Teraoka, S. Holler, and F. Vollmer, "Shift of whispering-gallery modes in microspheres by protein adsorption," Opt. Lett. 28, 272-274 (2003)

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