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Optics Letters

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  • Vol. 28, Iss. 6 — Mar. 15, 2003
  • pp: 414–416

Dual-axis confocal microscope for high-resolution in vivo imaging

Thomas D. Wang, Michael J. Mandella, Christopher H. Contag, and Gordon S. Kino  »View Author Affiliations


Optics Letters, Vol. 28, Issue 6, pp. 414-416 (2003)
http://dx.doi.org/10.1364/OL.28.000414


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Abstract

We describe a novel confocal microscope that uses separate low-numerical-aperture objectives with the illumination and collection axes crossed at angle θ from the midline. This architecture collects images in scattering media with high transverse and axial resolution, long working distance, large field of view, and reduced noise from scattered light. We measured transverse and axial (FWHM) resolution of 1.3 and 2.1μm, respectively, in free space, and confirm subcellular resolution in excised esophageal mucosa. The optics may be scaled to millimeter dimensions and fiber coupled for collection of high-resolution images in vivo.

© 2003 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.4730) Medical optics and biotechnology : Optical pathology

Citation
Thomas D. Wang, Michael J. Mandella, Christopher H. Contag, and Gordon S. Kino, "Dual-axis confocal microscope for high-resolution in vivo imaging," Opt. Lett. 28, 414-416 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-6-414


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References

  1. J. Pawley, ed., Handbook of Biological Confocal Microscopy, 3rd ed. (Plenum, New York, 1996).
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  3. M. Rajadhyaksha, R. R. Anderson, and R. H. Webb, Appl. Opt. 38, 2105 (1999).
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