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Optics Letters

Optics Letters


  • Vol. 29, Iss. 1 — Jan. 1, 2004
  • pp: 71–73

In vivo fluorescence microscopy of neuronal activity in three dimensions by use of voltage-sensitive dyes

Jonathan A. N. Fisher, Eugene F. Civillico, Diego Contreras, and Arjun G. Yodh  »View Author Affiliations

Optics Letters, Vol. 29, Issue 1, pp. 71-73 (2004)

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We report in vivo imaging of neuronal electrical activity from superficial layers of the mouse barrel cortex. The measurements have ∼16-μm spatial and 3-ms temporal resolution and reach depths of 150 μm below the cortical surface. The depth-dependent differential-fluorescence optical sections of activity are consistent with known cortical architecture and represent an important step toward in vivo measurement of functioning complex neural networks. Our observations employ a custom gradient-index lens probe and voltage-sensitive dye fluorescence; the use of epi-illumination rather than dark-field illumination provides the dramatic signal-to-noise improvement necessary for fast three-dimensional imaging.

© 2004 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

Jonathan A. N. Fisher, Eugene F. Civillico, Diego Contreras, and Arjun G. Yodh, "In vivo fluorescence microscopy of neuronal activity in three dimensions by use of voltage-sensitive dyes," Opt. Lett. 29, 71-73 (2004)

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