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Optics Letters

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  • Vol. 29, Iss. 1 — Jan. 1, 2004
  • pp: 77–79

In vivo flow cytometer for real-time detection and quantification of circulating cells

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin  »View Author Affiliations


Optics Letters, Vol. 29, Issue 1, pp. 77-79 (2004)
http://dx.doi.org/10.1364/OL.29.000077


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Abstract

An <i>in vivo</i> flow cytometer is developed that allows the real-time detection and quantification of circulating fluorescently labeled cells in live animals. A signal from a cell population of interest is recorded as the cells pass through a slit of light focused across a blood vessel. Confocal detection of the excited fluorescence allows continuous monitoring of labeled cells in the upper layers of scattering tissue, such as the skin. The device is used to characterize the <i>in vivo</i> kinetics of red and white blood cells circulating in the vasculatúre of the mouse ear. Potential applications in biology and medicine are discussed.

© 2004 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy

Citation
J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, "In vivo flow cytometer for real-time detection and quantification of circulating cells," Opt. Lett. 29, 77-79 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-1-77


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References

  1. H. M. Shapiro, Practical Flow Cytometry, 3rd ed. (Wiley-Liss, New York, 1995).
  2. J. P. Pawley, ed., Handbook of Biological Confocal Microscopy, 2nd ed. (Plenum, New York, 1995).
  3. Z. Chen, T. F. Milner, S. Srinivas, X. Wang, A. Malekafzali, J. C. Martin, and J. C. Nelson, Opt. Lett. 22, 1119 (1997).
  4. G. A. Thibodeau and K. A. Patton, Anatomy and Physiology, 4th ed. (Mosby, St. Louis, Mo., 1999).
  5. S. Basu, G. Hodgson, M. Katz, and A. R. Dunn, Blood 100, 854 (2002).
  6. G. G. Wulf, K.-L. Luo, M. A. Goodell, and M. K. Brenner, Blood 101, 2434 (2003).

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