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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 29, Iss. 11 — Jun. 1, 2004
  • pp: 1227–1229

Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, and Minoru Obara  »View Author Affiliations


Optics Letters, Vol. 29, Issue 11, pp. 1227-1229 (2004)
http://dx.doi.org/10.1364/OL.29.001227


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Abstract

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (~43°C) was effective in improving the transfection efficiency.

© 2004 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(140.3540) Lasers and laser optics : Lasers, Q-switched
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.7170) Medical optics and biotechnology : Ultrasound

Citation
Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, and Minoru Obara, "Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave," Opt. Lett. 29, 1227-1229 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-11-1227

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