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Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

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Abstract

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (43 °C) was effective in improving the transfection efficiency.

© 2004 Optical Society of America

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