Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (~43°C) was effective in improving the transfection efficiency.
© 2004 Optical Society of America
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(140.3540) Lasers and laser optics : Lasers, Q-switched
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.7170) Medical optics and biotechnology : Ultrasound
Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, and Minoru Obara, "Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave," Opt. Lett. 29, 1227-1229 (2004)