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Optics Letters

Optics Letters


  • Vol. 29, Iss. 12 — Jun. 15, 2004
  • pp: 1354–1356

Two-color two-photon 4Pi fluorescence microscopy

Jianfang Chen and Katsumi Midorikawa  »View Author Affiliations

Optics Letters, Vol. 29, Issue 12, pp. 1354-1356 (2004)

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In 4Pi fluorescence microscopy the point-spread function is composed of a strong central lobe accompanied by interference sidelobes that produce artifacts in the image. We propose to combine two-color two-photon fluorescence microscopy and 4Pi fluorescence microscopy to overcome this sidelobe problem. Simulation results show that a single sharp fluorescence spot can be produced by use of two excitation wavelengths of 400 and 800 nm and detected at 350-nm emission wavelength.

© 2004 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(320.0320) Ultrafast optics : Ultrafast optics
(350.5730) Other areas of optics : Resolution

Jianfang Chen and Katsumi Midorikawa, "Two-color two-photon 4Pi fluorescence microscopy," Opt. Lett. 29, 1354-1356 (2004)

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