OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 29, Iss. 12 — Jun. 15, 2004
  • pp: 1354–1356

Two-color two-photon 4Pi fluorescence microscopy

Jianfang Chen and Katsumi Midorikawa  »View Author Affiliations


Optics Letters, Vol. 29, Issue 12, pp. 1354-1356 (2004)
http://dx.doi.org/10.1364/OL.29.001354


View Full Text Article

Acrobat PDF (811 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In 4Pi fluorescence microscopy the point-spread function is composed of a strong central lobe accompanied by interference sidelobes that produce artifacts in the image. We propose to combine two-color two-photon fluorescence microscopy and 4Pi fluorescence microscopy to overcome this sidelobe problem. Simulation results show that a single sharp fluorescence spot can be produced by use of two excitation wavelengths of 400 and 800 nm and detected at 350-nm emission wavelength.

© 2004 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(320.0320) Ultrafast optics : Ultrafast optics
(350.5730) Other areas of optics : Resolution

Citation
Jianfang Chen and Katsumi Midorikawa, "Two-color two-photon 4Pi fluorescence microscopy," Opt. Lett. 29, 1354-1356 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-12-1354

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited