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Optics Letters

Optics Letters

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  • Vol. 29, Iss. 12 — Jun. 15, 2004
  • pp: 1396–1398

Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent

Changhuei Yang, Michael A. Choma, Laura E. Lamb, John D. Simon, and Joseph A. Izatt  »View Author Affiliations


Optics Letters, Vol. 29, Issue 12, pp. 1396-1398 (2004)
http://dx.doi.org/10.1364/OL.29.001396


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Abstract

We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and 83μM of phyA.

© 2004 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(300.6390) Spectroscopy : Spectroscopy, molecular
(350.5130) Other areas of optics : Photochemistry

Citation
Changhuei Yang, Michael A. Choma, Laura E. Lamb, John D. Simon, and Joseph A. Izatt, "Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent," Opt. Lett. 29, 1396-1398 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-12-1396


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