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Optics Letters

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  • Vol. 29, Iss. 13 — Jul. 1, 2004
  • pp: 1509–1511

Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy

Christopher G. Rylander, Digant P. Davé, Taner Akkin, Thomas E. Milner, Kenneth R. Diller, and Ashley J. Welch  »View Author Affiliations


Optics Letters, Vol. 29, Issue 13, pp. 1509-1511 (2004)
http://dx.doi.org/10.1364/OL.29.001509


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Abstract

We describe a method for <i>en face</i> phase-contrast imaging of cells with a fiber-based differential phase-contrast optical coherence microscopy system. Recorded <i>en face</i> images are quantitative phase-contrast maps of cells due to spatial variation of the refractive index and (or) thickness of various cellular components. Quantitative phase-contrast images of human epithelial cheek cells obtained with the fiber-based differential phase-contrast optical coherence microscopy system are presented.

© 2004 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy
(180.3170) Microscopy : Interference microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

Citation
Christopher G. Rylander, Digant P. Davé, Taner Akkin, Thomas E. Milner, Kenneth R. Diller, and Ashley J. Welch, "Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy," Opt. Lett. 29, 1509-1511 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-13-1509


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